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Biological Aging: Methods and Protocols de Trygve O. Tollefsbol

Biological Aging: Methods and Protocols: Methods in Molecular Biology, cartea 1048

Editat de Trygve O. Tollefsbol
en Limba Engleză Paperback – 30 apr 2017
The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.
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Specificații

ISBN-13: 9781493963133
ISBN-10: 1493963139
Pagini: 354
Ilustrații: XI, 354 p. 54 illus., 35 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Greutate: 0.64 kg
Ediția:Softcover reprint of the original 2nd ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Cell Senescence Culturing Methods.- Digital Image Analysis of Cells Stained with the Senescence-Associated -Galactosidase Assay.- Analysis of Biomarkers of Caloric Restriction in Aging Cells.- Cell Sorting of Young and Senescent Cells.- Studying the Replicative Life Span of Yeast Cells.- Methods for Creating Mutations in C. elegans that Extend Lifespan.- Aging Studies in Drosophila Melanogaster.- The Use of Calorie Restriction Mimetics to Study Aging.- Using Somatic-Cell Nuclear Transfer to Study Aging.- Induction of Cellular Senescence by Oncogenic RAS.- Methods of Cellular Senescence Induction Using Oxidative Stress.- Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice.- Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors.- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration.- Exometabolomic Mapping of Caenorhabditis elegans – A Tool to Non-Invasively Investigate Aging.- Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing.- Gel Electrophoresis-based Proteomics of Senescent Tissues.- Single-Cell Semiconductor Sequencing.- Application of DNA Microarray Technology to Gerontological Studies.- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells.- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging.

Textul de pe ultima copertă

The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.

Caracteristici

Focuses on contemporary approaches and established protocols to study aging cell culture Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Recenzii

From the reviews:
"For research gerontologists, this book is a boon." -Doody's Book Review, Weighted Numerical Score:90 - 4 Stars
"This can be a useful book for biogerontologists. … Comprised of 25 generally well-written articles … this book covers various methods and protocols for studying cellular aging of human cells, yeast cells, and mice. … a welcome source of useful information for researchers intending to find their way into descriptive, analytical and interventive research in biogerontology." (Suresh Rattan, Biogerontology, Issue 9, 2008)