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Cardiac Gene Therapy: Methods and Protocols de Kiyotake Ishikawa

Cardiac Gene Therapy: Methods and Protocols: Methods in Molecular Biology, cartea 1521

Editat de Kiyotake Ishikawa
en Limba Engleză Hardback – 2 dec 2016
This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.
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Specificații

ISBN-13: 9781493965861
ISBN-10: 1493965867
Pagini: 286
Ilustrații: XI, 355 p. 93 illus., 78 illus. in color.
Dimensiuni: 178 x 254 x 21 mm
Greutate: 0.85 kg
Ediția:1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Current Methods in Cardiac Gene Therapy: Overview.- Silencing Genes in the Heart.- Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs.- Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro.- Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction.- Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9.- Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV).- Synthesis of Modified mRNA for Myocardial Delivery.- Exosomes-Based Gene Therapy for MicroRNA Delivery.- Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents.- Gene Transfer in Isolated Adult Cardiomyocytes.- Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells.- Gene Transfer to Rodent Hearts In Vivo.- Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery.- A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy.- Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques.- Direct Myocardial Injection of Vectors.- Selective Pressure-Regulate Retroinfusion for Gene Therapy Application in Ischemic Heart Disease.- Cardiac Gene Delivery Using Recirculating Devices.- Molecular Cardiac Surgery with Recirculating Delivery (MCARD): Procedure and Vector Transfer.- Gene Delivery for the Generation of Bioartificial Pacemaker.- Gene Therapy for Post-Infarction Ventricular Tachycardia.- MicroRNA Delivery Strategies to the Lung in a Model of Pulmonary Hypertension.- Inhaled Gene Transfer for Pulmonary Circulation.

Textul de pe ultima copertă

This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.

Caracteristici

Includes cutting-edge methods and protocols for the study of cardiac gene therapy Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras