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Characterization of Nanoparticles Intended for Drug Delivery de Scott E. McNeil

Characterization of Nanoparticles Intended for Drug Delivery: Methods in Molecular Biology, cartea 1682

Editat de Scott E. McNeil
en Limba Engleză Paperback – 18 mai 2018
This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>
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Specificații

ISBN-13: 9781493984602
ISBN-10: 1493984608
Ilustrații: XII, 256 p. 72 illus., 40 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.49 kg
Ediția:Softcover reprint of the original 2nd ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Evaluating Nanomedicines: Obstacles and Advancements.- Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test.- Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations.- Elemental Analysis in Biological Matrices Using ICP-MS.- PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection.- Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis.- Immunoelectron Microscopy for Visualization of Nanoparticles.- Imaging of Liposomes by Transmission Electron Microscopy.- Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties.- In Vitro Assessment of Nanoparticle Effects on Blood Coagulation.- In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells.- Assessing NLRP3 Inflammasome Activation by Nanoparticles.- Analysis of Complement Activation by Nanoparticles.- Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo.- Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles.- Analysis of Nanoparticle Adjuvant Properties In Vivo.- In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions.- Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct.- Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer.- Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition.

Textul de pe ultima copertă

This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“This book details the latest advances in methodology for determining physiochemical, immunological, and toxicological properties of nanoparticles for use in medicine. … The chapter author are the very qualified experts responsible for developing the majority of the protocols for nanoparticle characterization … . serves an audience of researchers in the scientific community, pharmaceutical drug developers, and regulatory agencies. … This is an excellent resource for understanding the unique challenges in the development and in vitro evaluation of nanoparticle-based therapeutic agents.” (Rachel R. Chennault, Doody’s Review Service, June, 2011)