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Chloroplast Biotechnology: Methods and Protocols de Pal Maliga

Chloroplast Biotechnology: Methods and Protocols: Methods in Molecular Biology, cartea 2317

Editat de Pal Maliga
en Limba Engleză Paperback – 24 mai 2021
This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights 
of applications in agriculture, industrial biotechnology and healthcare.  

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Paperback (2) 77368 lei  43-57 zile
  Springer Us – 24 mai 2021 77368 lei  43-57 zile
  Humana Press Inc. – 3 sep 2016 77862 lei  43-57 zile
Hardback (2) 121190 lei  43-57 zile
  Humana Press Inc. – 5 mar 2014 121190 lei  43-57 zile
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Specificații

ISBN-13: 9781071614747
ISBN-10: 1071614746
Ilustrații: XII, 369 p. 70 illus., 41 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.66 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

The Plastid Genomes of Flowering Plants: Essential Principles.- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences.- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.- Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering.- Nicotiana tabacum: An Update on PEG-Mediated Plastid Transformation.- Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures.- Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinases.- Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression.- Plastid Transformation in Tomato: A Vegetable Crop and Model Species.- Stable Plastid Transformation of Petunia for Studies in Basic Science.- Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials.- Plastid Transformation of Poplar: A Model for Perennial Trees.- Plastid Transformation in Lettuce (Lactuca sativa) by Biolistic DNA Delivery.- Plastid Transformation in Sugar Beet: An Important Industrial Crop.- A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii.- Regulated Chloroplast Gene Expression in Chlamydomonas.- Plastid Transformation in Physcomitrella patens: An Update.- Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L..-Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.

Textul de pe ultima copertă

This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights 
of applications in agriculture, industrial biotechnology and healthcare.  

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts