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Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols de Paul C. Guest

Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols: Methods in Molecular Biology, cartea 2138

Editat de Paul C. Guest
en Limba Engleză Paperback – 27 mar 2021
This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical and Preclinical Models forMaximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.
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Specificații

ISBN-13: 9781071604731
ISBN-10: 1071604732
Pagini: 451
Ilustrații: XIV, 451 p. 84 illus., 57 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.77 kg
Ediția:1st ed. 2020
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

The Impact of New Biomarkers and Drug Targets on Age-Related Disorders.- Vitamin D and Muscle Sarcopenia in Aging.- Quantitative Assays of Plasma Apolipoproteins.- Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice.- An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models.- Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations.- Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster.- Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors.- Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System.- Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells.- Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines.- Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans.- Clinical Assessment of the NADome as Biomarkers for Healthy Aging.- 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract.- Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms.- Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae.- Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging.- Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue.- Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency.- Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer.- Quantitative Analysis of DNA Methylation by Bisulfite Sequencing.- Histomorphometric Analysis of Anti-Aging Properties on Rat Skin.- Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat.- Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients.- Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women.- The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women.- Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction.- Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases.- Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach.- Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging.- Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine.- MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging.

Textul de pe ultima copertă

This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical and Preclinical Models forMaximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts