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Clinical Applications of Capillary Electrophoresis: Methods and Protocols: Methods in Molecular Biology, cartea 919

Editat de Terry M. Phillips, Heather Kalish
en Limba Engleză Hardback – 13 sep 2012
Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.
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Specificații

ISBN-13: 9781627030281
ISBN-10: 162703028X
Pagini: 470
Ilustrații: XIII, 311 p.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.77 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

An Overview of CE in Clinical Analysis.- Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection.- The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis – Mass Spectrometry.- Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids.- Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS).- Development of an |On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE.- Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D).- A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection.- High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems.- Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection.- ABO Genotyping by Capillary Electrophoresis.- Separation of Hemoglobin Variants by Capillary Electrophoresis.- Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis.- Application of CZE in the Differentiation of Staphylococcus Aureus Strains.- Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism.- Application of CGE to Virus Identification.- Capillary Electrophoresis-Based ProteomicTechniques for Biomarker Discovery.- Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis.- Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease.- Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker.- Chip-Based Immunoassays.- CE Analysis of g-Globulin Mobility and Potential Clinical Utility.- Cytokine Analysis by Immunoaffinity Capillary Electrophoresis.- Capillary Electrophoresis for the Detection of Fragile X Expanded Alleles.- Analysis of Microsatellite Instability by Microfluidic-Based Electrophoresis.- HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.

Textul de pe ultima copertă

Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts