Cantitate/Preț
Produs
Total produse
Vezi coșul
CRISPR Gene Editing: Methods and Protocols de Yonglun Luo

CRISPR Gene Editing: Methods and Protocols: Methods in Molecular Biology, cartea 1961

Editat de Yonglun Luo
en Limba Engleză Hardback – 26 mar 2019
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Citește tot Restrânge

Din seria Methods in Molecular Biology

  • 70% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 26096 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59855 lei
  • 19% Plant-Pathogen Interactions: Methods and Protocols
    Preț: 57771 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49678 lei
  • 20% Clinical Bioinformatics
    Preț: 88293 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 70873 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71049 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72009 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 72575 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 64446 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 99590 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 71354 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 88795 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 64558 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 63539 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 137406 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 72134 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 94440 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64158 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 71727 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 95666 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 71229 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39257 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 72490 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 93503 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86019 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 63395 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 102521 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79215 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42361 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42590 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59219 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 5% Synthetic Metabolic Pathways: Methods and Protocols
    Preț: 37270 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103882 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 206129 lei
  • 5% Epitope Mapping Protocols
    Preț: 126192 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78992 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133907 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 135031 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75265 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37488 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 135552 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 109722 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 136778 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 109261 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 93855 lei

Preț: 126422 lei

Preț vechi: 133076 lei
-5% Nou

Puncte Express: 1896
Preț estimativ în valută:
24194€ • 25446$ • 20153£

Carte tipărită la comandă

Livrare economică 04-18 ianuarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493991693
ISBN-10: 1493991698
Pagini: 367
Ilustrații: XI, 362 p. 76 illus., 61 illus. in color.
Dimensiuni: 178 x 254 x 29 mm
Greutate: 0.87 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

CRISPR-gRNA Design.- Tracking CRISPR’s Footprints.- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER.- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA.- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check.- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC).- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery.- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors.- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs.- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes.- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9.- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells.- Rapid and Efficient Gene Deletion by CRISPR/Cas9.- Genome Editing in Mice.- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol.- Gene Editing in Primary Cells of Cattle and Pig.- Toward In Vivo Gene Therapy Using CRISPR.- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery.- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection.- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.

Textul de pe ultima copertă

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts