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Yarrowia lipolytica: Methods and Protocols: Methods in Molecular Biology, cartea 2307

Editat de Ian Wheeldon, Mark Blenner
en Limba Engleză Paperback – 15 apr 2022
This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.
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Specificații

ISBN-13: 9781071614167
ISBN-10: 1071614169
Ilustrații: X, 250 p. 65 illus., 44 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.46 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Genome Engineering of Yarrowia lipolytica with the piggyBac Transposon System.- Genome Editing in Y. lipolytica Using TALENs.- EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica.- Simultaneous gene excision and integration by dual-guide CRISPR-Cas9.- Gene excision by dual-guide CRISPR-Cas9.- CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica..- Implementing CRISPR-Cas12a for Efficient Genome-editing in Yarrowia lipolytica.- Guide RNA Design for Genome-wide CRISPR Screens in Yarrowia lipolytica.- Targeted Integration through Transformation of Hydroxyurea-Arrested Cells.- Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica.- Engineering Yarrowia lipolytica for production of fatty alcohols with YaliBrick Vectors.- Methods to Activate and Elucidate ComplexEndogenous Sugar Metabolism in Yarrowia lipolytica.- Pathway Engineering For Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica.- Droplet-based Microfluidic High-throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica.- Yarrowia lipolytica Bioprocess Development – From Flask to Bioreactor.- Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica.



Textul de pe ultima copertă

This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  
Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts