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Crop Breeding: Genetic Improvement Methods: Methods in Molecular Biology, cartea 2264

Editat de Pasquale Tripodi
en Limba Engleză Paperback – 4 dec 2021
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs,  mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.

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Specificații

ISBN-13: 9781071612033
ISBN-10: 1071612034
Pagini: 280
Ilustrații: XI, 280 p. 62 illus., 54 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.51 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Methods of Development of Biparental Mapping Populations in Horticultural Crops.- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits.- Using Metabolomics to Assist Plant Breeding.- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications.- High Resolution Melting Analysis as a Tool for Plant Species Authentication.- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods.- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome.- Association Mapping in Plants.- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs).- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis.- RNA Interference (RNAi) in Tomato Crop Reseserch.- Protoplast Based Method for Genome Editing in Tetraploid Potato.- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria.- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant.- Assembly of TALEN and mTALE-Act for Plant Genome Engineering.- Genome Editing to Achieve the Crop Ideotype in Tomato.- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging.- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.


Textul de pe ultima copertă

This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs,  mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.


Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts