Cantitate/Preț
Produs
Total produse
Vezi coșul
Enzyme Engineering: Methods and Protocols de Francesca Magnani

Enzyme Engineering: Methods and Protocols: Methods in Molecular Biology, cartea 2397

Editat de Francesca Magnani, Chiara Marabelli, Francesca Paradisi
en Limba Engleză Paperback – 25 noi 2022
This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and timely, Enzyme Engineering: Methods and Protocols is an ideal guide for both the novice and the veteran researcher interested in biocatalysis.

Chapter 13 is available open access under a CC BY 4.0 license.

Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 77590 lei  6-8 săpt.
  Springer Us – 25 noi 2022 77590 lei  6-8 săpt.
Hardback (1) 110282 lei  6-8 săpt.
  Springer Us – 24 noi 2021 110282 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 60% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 34795 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59856 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49679 lei
  • 20% Clinical Bioinformatics
    Preț: 88295 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 71733 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71911 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72883 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 73457 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 65228 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 100802 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 72221 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 89877 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 65342 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 64310 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 139083 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 73010 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 95589 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64937 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 72598 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 96831 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 72093 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39258 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 73370 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 94642 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86021 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 64166 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 103769 lei
  • 23% Computational Protein Design
    Preț: 88385 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79216 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42362 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42591 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59220 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103884 lei
  • 5% Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications
    Preț: 52415 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 208644 lei
  • 5% Epitope Mapping Protocols
    Preț: 127730 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78993 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133910 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 136679 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75266 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37489 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 137205 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 111057 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 138447 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 110593 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 94998 lei

Preț: 77590 lei

Preț vechi: 94622 lei
-18% Nou

Puncte Express: 1164
Preț estimativ în valută:
14849€ • 15508$ • 12311£

Carte tipărită la comandă

Livrare economică 11-25 februarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781071618288
ISBN-10: 1071618288
Pagini: 360
Ilustrații: XI, 360 p. 84 illus., 69 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.65 kg
Ediția:1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Preparation of Soil Metagenome Libraries and Screening for Gene Specific Amplicons.- Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics.- Synthetic DNA Libraries for Protein Engineering toward Process Improvement in Drug Synthesis.- In Silico Prediction Methods for Site-Saturation Mutagenesis.- Recombination of Compatible Substitutions by 2GenReP and InSiReP.- Using the Evolutionary History of Proteins to Engineer Insertion-Deletion Mutants from Robust, Ancestral Templates Using Graphical Representation of Ancestral Sequence Predictions (GRASP).- Resurrecting Enzymes by Ancestral Sequence Reconstruction.- Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactors.- Rational-Design Engineering to Improve Enzyme Thermostability.- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents.- In Silico Engineering of Enzyme Access Tunnels.- Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamics.- Computational Enzyme Design at Zymvol.- Advanced Enzyme Immobilization Technologies: An Eco-Friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-Immobilization Technique.- Chemical Reaction Engineering to Understand Applied Kinetics in Free Enzyme Homogeneous Reactors.- Enzymatic Oxidative Cascade for Oxofunctionalization of Fatty Acids in One-Pot.- CRISPR-Cas9 Editing of the Synthesis of Biodegradable Polyesters Polyhydroxyalkanaotes (PHA) in Pseudomonas putida KT2440.

Textul de pe ultima copertă

This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and timely, Enzyme Engineering: Methods and Protocols is an ideal guide for both the novice and the veteran researcher interested in biocatalysis.

Chapter 13 is available open access under a CC BY4.0 license.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts