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Epithelial Cell Culture: Methods and Protocols: Methods in Molecular Biology, cartea 2749

Editat de Mario Baratta
en Limba Engleză Hardback – 23 dec 2023
Back Cover Copy
This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell biology, and the cutting-edge research and techniques used by researchers in the field. The chapters in this book cover topics such as detailed methodologies applicable to epithelial cells derived from primates, pigs, bovines, and laboratory animals; the manipulation and differentiation of epithelial cells; and epithelial cell models in the gastroenteric system in human medicine and nutrition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Epithelial Cell Culture: Methods and Protocols, Second Edition is a valuable resource for researchers in the scientific community, educators,and students who are interested in unraveling the complexities of epithelial cell biology, cultivating curiosity, and inspiring the next generation of groundbreaking research.



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Specificații

ISBN-13: 9781071636084
ISBN-10: 1071636081
Pagini: 186
Ilustrații: XI, 186 p. 44 illus., 39 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.57 kg
Ediția:2nd ed. 2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium.- Cultured Pig Thyroid Follicular Cells: Electrical Evaluation of Epithelial Integrity.- Human Minor Salivary Glands: A Readily-Available Source of Salivary Stem/Progenitor Cells for Regenerative Applications.- Salivary Organotypic Tissue Culture: An Ex-Vivo 3D Model for Studying Radiation-Induced Injury of Human Salivary Glands.- Differentiation of Pig Gastric Primary Cells into Mucus Producing Epithelial Cells.- Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells.- Method for Two-Dimensional Epithelial Monolayer Formation Derived from Mouse Three-Dimensional Small Intestinal Organoids.- Human Hepatic Spheroid Co-Culture Model for Assessment of Drug-Induced Liver Injury.- In Vitro Porcine (Explant) Colon Culture.- Bioencapsulation of Oocytes and Granulosa Cells.- Culturing and Differentiation of Patient-Derived Ectocervical EpithelialStem Cells using Air-Liquid Interphase and Matrigel Scaffold.- Ovine Trophoblast Cells: Cell Isolation and Culturing from the Placenta at the Early Stage of Pregnancy.- Amniotic Membrane and Amniotic Epithelial Cell Culture.- Evaluation of the Epithelial Barrier Integrity in Primary Cultures of Pig Mammary Epithelial Cells.- Bovine Skeletal Muscle Satellite Cells: Isolation, Growth, and Differentiation.- Equine Induced Pluripotent Stem Cell Culture.


Textul de pe ultima copertă

Back Cover Copy
This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell biology, and the cutting-edge research and techniques used by researchers in the field. The chapters in this book cover topics such as detailed methodologies applicable to epithelial cells derived from primates, pigs, bovines, and laboratory animals; the manipulation and differentiation of epithelial cells; and epithelial cell models in the gastroenteric system in human medicine and nutrition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Epithelial Cell Culture: Methods and Protocols, Second Edition is a valuable resource for researchers in the scientific community, educators,and students who are interested in unraveling the complexities of epithelial cell biology, cultivating curiosity, and inspiring the next generation of groundbreaking research.




Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Notă biografică

Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.