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Exon Skipping and Inclusion Therapies: Methods and Protocols de Toshifumi Yokota

Exon Skipping and Inclusion Therapies: Methods and Protocols: Methods in Molecular Biology, cartea 1828

Editat de Toshifumi Yokota, Rika Maruyama
en Limba Engleză Hardback – sep 2018
This book presents a comprehensive collection of detailed state-of-the-art exon skipping and splices modulation protocols. Chapters detail 14 genetic diseases, AON-mediated therapies, and CRISPR/Cas9-mediated gene editing therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Exon Skipping and Inclusion Therapies: Methods and Protocols aims to help researchers initiate the development of next-generation therapies.
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Specificații

ISBN-13: 9781493986507
ISBN-10: 1493986503
Pagini: 530
Ilustrații: XV, 569 p. 103 illus., 77 illus. in color.
Dimensiuni: 178 x 254 x 40 mm
Greutate: 1.23 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Invention and Early History of Exon Skipping and Splice Modulation.- An Overview of Recent Advances and Clinical Applications of Exon Skipping and Splice Modulation for Muscular Dystrophy and Various Genetic Diseases.- Recent Advances and Clinical Applications of Exon Inclusion for Spinal Muscular Atrophy.- Nusinersen in the Treatment of Spinal Muscular Atrophy.- Tips to Design Effective Splice-switching Antisense Oligonucleotides for Exon Skipping and Exon Inclusion.- Antisense Oligonucleotide Targeting of 3’UTR of mRNA for Expression Knockdown.- Quantitative Evaluation of Exon Skipping in Immortalized Muscle Cells In Vitro.- Direct Reprogramming of Human DMD Fibroblasts into Myotubes for In Vitro Evaluation of Antisense-m Exon Skipping and Exons 45-55 Skipping Accompanied by Rescue of Dystrophin Expression.- In vitro Multi-exon Skipping by Antisense PMOs in Dystrophic Dog and Exon 7-Deleted DMD Patient.- Creation of DMD Muscle Cell Model using CRISPR-Cas9 Genome Editing toTest the Efficacy of Antisense-mediated Exon Skipping.- In vitro Evaluation of Exon Skipping in Disease Specific iPSC-derived Myocytes.- Restoration of Dystrophin Protein Expression by Exon Skipping utilizing CRISPR-Cas9 in Myoblasts Derived from DMD Patient iPS Cells.- Skipping of Duplicated Dystrophin Exons: in vitro Induction and Assessment.- In Vivo Evaluation of Dystrophin Exon Skipping in mdx Mice.- Exon 51 Skipping Quantification by Digital Droplet PCR in del52hDMD/mdx Mice.- Systemic Injection of Peptide-PMOs into Humanized DMD Mice and Detection by RT-PCR and ELISA.- In vivo Evaluation of Single- and Multi-exon Skipping in mdx52 Mice.- A Novel Zebrafish Model for Assessing In Vivo Delivery of Morpholino Oligomers.- Validation and Detection of Exon Skipping Boosters in DMD Patient Cell Models and mdx Mouse.- Use of Glucose/Fructose to Enhance the Exon Skipping Efficacy.- Systemic Intravenous Administration of Antisense Therapeutics forCombinatorial Dystrophin and Myostatin Exon Splice Modulation.- The Assembly of Fluorescently Labeled Peptide-oligonucleotide Conjugates via Orthogonal Ligation Strategies.- In vivo Evaluation of Multiple Exon Skipping with Peptide-PMOs in Cardiac and Skeletal Muscles in Dystrophic Dogs.- Use of Tricyclo-DNA Antisense Oligonucleotides for Exon Skipping.- Optimization of 2ʹ,4ʹ-BNA/LNA–based Oligonucleotides For Splicing Modulation in vitro.- Pre-Mrna Splicing Modulation by Antisense Oligonucleotides.- In vitro Evaluation of Antisense-mediated Exon Inclusion for Spinal Muscular Atrophy.- Systemic Injection of Antisense oligos into SMA Mice and Evaluation.- Exon Skipping using Antisense Oligonucleotides for Laminin-alpha2-deficient Muscular Dystrophy.- Exon Skipping by Ultrasound-enhanced Delivery of Morpholino with Bubble Liposomes for Myotonic Dystrophy Model Mice.- Dysferlin Exon 32 Skipping in Patient Cel­ls.- Morpholino-mediated Exon Skipping Targeting Human ACVR1/ALK2 for Fibrodysplasia Ossificans Progressiva.- Exon Skipping of FcεRIβ for Allergic Diseases.- Antisense Oligonucleotide Design and Evaluation of Splice-modulating Properties Using Cell-based Assays.- Antisense-mediated Splice Modulation to Reframe Transcripts.- Morpholino-mediated Exon Inclusion for SMA. 

Textul de pe ultima copertă

This book presents a comprehensive collection of detailed state-of-the-art exon skipping and splices modulation protocols. Chapters detail 14 genetic diseases, AON-mediated therapies, and CRISPR/Cas9-mediated gene editing therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Exon Skipping and Inclusion Therapies: Methods and Protocols aims to help researchers initiate the development of next-generation therapies.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts