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Flow Cytometry Protocols: Methods in Molecular Biology, cartea 699

Editat de Teresa S. Hawley, Robert Hawley
en Limba Engleză Hardback – dec 2010
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.
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Specificații

ISBN-13: 9781617379499
ISBN-10: 1617379492
Pagini: 465
Ilustrații: XII, 485 p.
Dimensiuni: 178 x 254 x 33 mm
Greutate: 1.06 kg
Ediția:3rd ed. 2011
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Flow Cytometry: An Introduction.- Breaking the Dimensionality Barrier.- Quantitative Fluorescence Measurements with Multicolor Flow Cytometry.- Quantum Dots for Quantitative Flow Cytometry.- Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry.- Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization.- Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry.- Multiparameter Intracellular Cytokine Staining.- Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Multiparameter Cell Cycle Analysis.- Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue.- Flow Cytometric-Based Identification of Immature Myeloerythroid Development.- Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia.- Flow Cytometry Assays in Primary Immunodeficiency Diseases.- Flow Cytometric Analysis of Microparticles.- Noncytotoxic DsRed Derivatives for Whole-Cell Labeling.- Flow Cytometric FRET Analysis of Protein Interaction.- Fluorescent Protein-Assisted Purification for Gene Expression Profiling.- Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type.- Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics.- Standard Practice for Cell Sorting in a BSL-3 Facility.- The Cytometric Future: It Ain’t Necessarily Flow!

Textul de pe ultima copertă

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

Caracteristici

Features polychromatic, quantitative, and high throughput flow cytometry protocols applicable to basic biological research as well as diagnostic medicine Paves the way for flow cytometry's use in the study of systems biology Includes extensive expert notes to ensure optimal results Includes supplementary material: sn.pub/extras

Recenzii

"This is a valuable addition to the literature and will be of benefit to research scientists and clinicians whether they be experienced or new to the methodology." - Cell Biochemistry and Function

From Reviews of the First Edition...
"...a well and precisely written collection of protocols which provide important information for both experienced and novice flow cytometry users." -FEBS Letters

"...There are many people who would benefit from reading this book, from those currently using cytometry...to molecular biologists interested in looking at DNA or RNA in the context of cell phenotype in mixed cell populations and those involved in diagnostic testing..." -Molecular Biotechnology