Glycoprotein Analysis in Biomedicine: Methods in Molecular Biology, cartea 14

A General Strategy for Glycoprotein Oligosaccharide Analysis.- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography.- Sequence and Linkage Analysis of N-and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry.- Gas Chromatography and Gas Chromatography-Mass Spectrometry of Glycoprotein Oligosaccharides.- Microscale Sequencing of N-Linked Oligosaccharides of Glycoproteins Using Hydrazinolysis, Bio-Gel P-4, and Sequential Exoglycosidase Digestion.- Analysis of Sugar Chains by Pyridylamination.- Analysis of Carbohydrates in Glycoproteins by High-Performance Liquid Chromatography and High-Performance Capillary Electrophoresis.- The Microanalysis of Glycosyl-Phosphatidylinositol Glycans.- Analysis of Bacterial Glycoproteins.- Isolation and Fractionation of Glycoprotein Glycans in Small Amounts.- High-Mannose Chains of Mammalian Glycoproteins.- Release of O-Linked Glycoprotein Glycans by Endo-?-N-Acetylgalactosaminidase.- Immunological Detection of Glycoproteins on Blots Based on Labeling with Digoxigenin.- Glycosyltransferases as Tools in Cell Biological Studies.- Purification of the EGF Receptor for Oligosaccharide Studies.- Analysis of Sulfated Polysaccharide Conjugates.- Secreted Mucus Glycoproteins in Cell and Organ Culture.- Preparation of Polyclonal Antibodies to Native and Modified Mucin Antigens.- The Use of Immunoperoxidase to Characterize Gastrointestinal Mucin Epitopes.- The Application of Lectins to the Study of Mucosal Glycoproteins.- Detection of Tumor-Associated Expression of Carbohydrate-Binding Proteins (Lectins).- Scanning Tunneling Microscopy of Biopolymers.

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