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Peptide Modifications to Increase Metabolic Stability and Activity: Methods in Molecular Biology, cartea 1081

Editat de Predrag Cudic
en Limba Engleză Hardback – 7 sep 2013
In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities. These methods and protocols include preparation of conformationally constrained peptides, modification of peptide bonds, introduction of nonproteinogenic amino acids, and alteration of peptides’ physical and biological properties by modification of the amino acid side chains and/or terminal residues. With additional chapter that describes new experimental approach for the detection of exogenous peptides within living cells using peptides labeled with heavy isotopes and confocal Raman microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Peptide Modifications to Increase Metabolic Stability and Activity seeks to provide scientists with alternative approaches to peptide modification that many researchers may find applicable to their specific research requirements.
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Specificații

ISBN-13: 9781627036511
ISBN-10: 1627036512
Pagini: 252
Ilustrații: IX, 241 p. 92 illus., 16 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Greutate: 0.64 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Hantzsch Based Macrocyclization Approach for the Synthesis of Thiazole Containing Cyclopeptides.- The Chemical Synthesis of α-Conotoxins and Structurally Modified Analogs with Enhanced Biological Stability.- Synthesis of AApeptides.- Peptoids and Peptide – Peptoid Hybrid Biopolymers as Peptidomimetics.- Synthesis of Side Chain N,N′-diaminoalkylated Derivatives of Basic Amino Acids for Application in Solid-phase Peptide Synthesis.- Study Protein Folding and Aggregation Using Non-natural Amino Acid p-cyanophenylalanine as a Sensitive Optical Probe.- AdamantoyLated Biologically Active Small Peptides and Glycopeptides Structurally Related to the Bacterial Peptidoglycan.- Optimization of Physicochemical and Pharmacological Properties of Peptide Drugs by Glycosylation.- The Maillard Reaction Induced Modification of Endogenous Opioid Peptide Encephalin.- Solid-phase Guanidinylation of Peptidyl Amines Compatible with Standard Fmoc-chemistry: Formation of Monosubstituted Guanidines.- Stabilization of Collagen-Model, Triple-Helical Peptides for In Vitro and In Vivo Applications.- Identification of Adipokine Receptor Agonists and Turning Them to Antagonists.- Peptide Detection and Structure Determination in Live Cells using Confocal Raman.

Textul de pe ultima copertă

In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities. These methods and protocols include preparation of conformationally constrained peptides, modification of peptide bonds, introduction of nonproteinogenic amino acids, and alteration of peptides’ physical and biological properties by modification of the amino acid side chains and/or terminal residues. With additional chapter that describes new experimental approach for the detection of exogenous peptides within living cells using peptides labeled with heavy isotopes and confocal Raman microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Peptide Modifications to Increase Metabolic Stability and Activity seeks to provide scientists with alternative approaches to peptide modification that many researchers may find applicable to their specific research requirements.

Caracteristici

Summarizes methods for preparation and purification of modified peptides Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts