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Handbook of ELISPOT: Methods and Protocols de Alexander E. Kalyuzhny

Handbook of ELISPOT: Methods and Protocols: Methods in Molecular Biology, cartea 1808

Editat de Alexander E. Kalyuzhny
en Limba Engleză Paperback – 10 dec 2019
This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”
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Specificații

ISBN-13: 9781493993284
ISBN-10: 1493993283
Pagini: 251
Ilustrații: X, 251 p. 74 illus., 68 illus. in color.
Dimensiuni: 178 x 254 x 23 mm
Ediția:Softcover reprint of the original 3rd ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Challenges in Developing Protein Secretion Assays at a Single-Cell Level.- To Count of Not to Count: ELISPOT Evaluation Revisited.- Essential Controls for ELISpot Assay.- Automatic Search of Spots and Color Classification in ELISPOT Assay.- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages.- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay.- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays.- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite.- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT.- ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections.- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection.- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins.- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines.- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.

Textul de pe ultima copertă

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
"The book is divided into four sections, with a total of 20 chapters featuring step-by-step protocols that will enable both novice and experienced researchers to perform ELISPOT. Each chapter begins with a general Introduction, followed by a detailed Materials section and an easy-to-follow Methods section … . All the chapters are equally interesting and well presented … . a must for a cytochemist. This book will serve as a convenient reference manual for beginners and for experienced ELISPOT users." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)