High Content Screening (Methods in Molecular Biology) de Paul A. Johnston

High Content Screening: A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery: Methods in Molecular Biology, cartea 1683

Paul A. Johnston

Oscar J. Trask

en Limba Engleză Paperback – 30 aug 2018

This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field.

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Cuprins

Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays.- General Staining and Segmentation Procedures for High Content Imaging and Analysis.- Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis.- Cell-based High Content Analysis of Cell Proliferation and Apoptosis.- Tools to Measure Autophagy using High Content Imaging and Analysis.- Guidelines for Microplate Selection in High Content Imaging.- Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler.- High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening.- Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays.- Live-Cell High Content Screening in Drug Development.- Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening.- Translocation Biosensors - Versatile Tools to Probe ProteinFunctions In Living Cells.- High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions.- High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines.- Single Cell and Population Level Analysis of HCA Data.- Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens.- High Content Screening of Mammalian Primary Cortical Neurons.- Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications.- Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays.- The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates.- An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro.- High-throughput Automated Chemical Screens in Zebrafish.

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