Cantitate/Preț
Produs

Mammalian Cell Viability: Methods and Protocols: Methods in Molecular Biology, cartea 740

Editat de Martin J. Stoddart
en Limba Engleză Hardback – 12 apr 2011
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
 
Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 76100 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 76100 lei  6-8 săpt.
Hardback (1) 63513 lei  6-8 săpt.
  Humana Press Inc. – 12 apr 2011 63513 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 63513 lei

Preț vechi: 74721 lei
-15% Nou

Puncte Express: 953

Preț estimativ în valută:
12154 12816$ 10118£

Carte tipărită la comandă

Livrare economică 11-25 ianuarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781617791079
ISBN-10: 1617791075
Pagini: 252
Ilustrații: XI, 240 p.
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.64 kg
Ediția:2011
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Cell Viability Assays: Introduction.- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods.- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay.- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells.- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye.- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability.- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays.- Annexin V / 7-AAD Staining in Keratinocytes.- Measurement of Caspase Activity: From Cell Populations to Individual Cells.- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes.- Multiplexing Cell Viability Assays.- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death.- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs.- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers.- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections.- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay.- Exploiting the Liberation of Zn2+ to Measure Cell Viability.- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs.- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test.- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses.-Digital Image Processing of Live/Dead Staining.

Textul de pe ultima copertă

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
 
Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Caracteristici

Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment Facilitates the multiplexing of methods for the greatest levels of success Contains expert tips and key implementation advice