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Microarray Methods for Drug Discovery: Methods in Molecular Biology, cartea 632

Editat de Sridar V. Chittur
en Limba Engleză Paperback – 23 aug 2016

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Specificații

ISBN-13: 9781493957392
ISBN-10: 1493957392
Pagini: 285
Ilustrații: XI, 285 p.
Dimensiuni: 178 x 254 mm
Greutate: 0.52 kg
Ediția:Softcover reprint of the original 1st ed. 2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Multicenter Clinical Sample Collection for Microarray Analysis.- Isolation of Total RNA from Transgenic Mouse Melanoma Subsets Using Fluorescence-Activated Cell Sorting.- Microarray Analysis of Embryonic Stem Cells and Differentiated Embryoid Bodies.- Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST Arrays.- Profiling microRNA Expression with the Illumina BeadChip Platform.- TaqMan® Array Cards in Pharmaceutical Research.- DMET™ Microarray Technology for Pharmacogenomics-Based Personalized Medicine.- The Use of Microarray Technology for Cytogenetics.- PCR/LDR/Universal Array Platforms for the Diagnosis of Infectious Disease.- RIP-CHIP in Drug Development.- ChIPing Away at Global Transcriptional Regulation.- HELP (HpaII Tiny Fragment Enrichment by Ligation-Mediated PCR) Assay for DNA Methylation Profiling of Primary Normal and Malignant B Lymphocytes.- High-Throughput Screening of Metalloproteases Using Small Molecule Microarrays.- Metabolic Enzyme Microarray Coupled with Miniaturized Cell-Culture Array Technology for High-Throughput Toxicity Screening.- Use of Tissue Microarray to Facilitate Oncology Research.- Small Molecule Selectivity and Specificity Profiling Using Functional Protein Microarrays.- Production and Application of Glycan Microarrays.

Recenzii

From the reviews:
“Microarray Methods for Drug Discovery is volume number 632 of the Springer Protocols Methods in Biology Series. … it provides detailed experimental protocols for the use of microarrays in a variety of applications. … chapters are obviously highly bespoke and would be of real value to laboratories wishing to establish the method. Finally, the microarray area is now so broad that the non-nucleic acid application of microarrays would justify a methods book in its own right.” (James Sidaway, BTS Newsletter, Issue 37, Winter, 2010)
“Methods in Microarrays provide scientists with a complete resource on the most effective tools available for maximizing microarray data in biochemical methods. … Microarrays are extensively used in the effects of compounds on gene expression in cells. This book aims at enlightening individuals of all levels of experience about some of the most common applications of microarrays in drug discovery and development. This book will serve as a reference for students and scientists … .” (Veni Maria Andres Felli, Brazilian Journal of Pharmaceutical Sciences, May, 2010)

Textul de pe ultima copertă

While the utilization of microarrays for gene expression studies has been widely published, Microarray Methods for Drug Discovery describes the use of this technology for a multitude of other vital applications. Covering the field of drug discovery through its various aspects, including high throughput screening, target identification, drug metabolism and toxicity screening as well as clinical sample handling, this volume also addresses new emerging fields like miRNA profiling, ribonomic and glycomic profiling. All chapters, contributed by experts in the field, follow the highly successful Methods in Molecular Biology™ series format, which is comprised of brief introductions, lists of required materials and reagents, readily reproducible, step-by-step lab protocols, along with detailed tips on troubleshooting and avoiding known pitfalls, making this book an easy read for investigators at all levels of expertise.
Authoritative and cutting-edge, Microarray Methods for Drug Discovery provides an ideal desk reference for scientists who wish to use microarray technology in order to better understand a variety of biological interactions in their respective fields.

Caracteristici

Supplies new information on microarrays for cytogenetics and drug metabolism Covers a wide range of microarray platforms used in genomics, epigenomics, genotyping, proteomics, and small molecule screening A good reference for designing microarray experiments to decipher transcriptome regulation and DNA modifications Includes supplementary material: sn.pub/extras