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Microbial Systems Biology: Methods and Protocols de Ali Navid

Microbial Systems Biology: Methods and Protocols: Methods in Molecular Biology, cartea 2349

Editat de Ali Navid
en Limba Engleză Hardback – 31 oct 2021
This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.
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Hardback (2) 95522 lei  6-8 săpt.
  Humana Press Inc. – 27 mai 2012 95522 lei  6-8 săpt.
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Specificații

ISBN-13: 9781071615843
ISBN-10: 107161584X
Pagini: 385
Ilustrații: XII, 385 p. 105 illus., 71 illus. in color.
Dimensiuni: 178 x 254 x 32 mm
Greutate: 0.86 kg
Ediția:2nd ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14 Labeled Analytes.- Fast sampling of the cellular metabolome.- Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry.- Western Blot Processing Optimization: The Perfect Blot.- FISHing on a Budget.- NanoSIP: NanoSIMS Applications for Microbial Biology.- Construction of Metatranscriptomic Libraries for 5' end Sequencing of rRNAs for Microbiome Research.- Computational Approaches for Designing Highly Specific and Efficient sgRNAs.- Complex Network Analysis in Microbial Systems: Theory and Examples.- Prokaryotic Genome Annotation.- Functional Annotation from Structural Homology.- Metabolic Modeling with MetaFlux.- Application of the Metabolic Modeling Pipeline in KBase to Categorize Reactions, Predict Essential Genes, And Predict Pathways in an Isolate Genome.- Curating COBRA Models of Microbial Metabolism.- A Beginner’s Guide to the COBRA Toolbox.- Rules of Engagement: A Guide to Developing Agent-Based Models.

Textul de pe ultima copertă

This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  
Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“This book … deals with one of the most dynamic trends in Biology, namely Systems Biology of Microorganisms. … Most of the chapters are of direct and immediate usefulness for scientists to start their own experiments with respect to different aspects of systems biology of a given microorganism or community/environment. … In my opinion, the purpose of this book … to introduce the reader to some of the established and state-of-the-art technologies that are being used to study microbial systems biology – is fully accomplished.” (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)