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MicroRNA and Cancer: Methods and Protocols: Methods in Molecular Biology, cartea 1699

Editat de Wei Wu
en Limba Engleză Paperback – 23 aug 2018
This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field
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Specificații

ISBN-13: 9781493984855
ISBN-10: 1493984853
Pagini: 222
Ilustrații: XI, 222 p. 21 illus., 17 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:Softcover reprint of the original 2nd ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics.- MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential.- Involvement of miRNAs and Pseudogenes in Cancer.- microRNAs Reprogram Tumor Immune Response.- APOBEC Gene Expression Regulated by microRNAs.- MicroRNAs Changes the Landscape of Cancer Resistance.- MicroRNA, Noise, and Gene Expression Regulation.- Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer.- Detection of Plasma microRNA Signature in Osteosarcoma Patients.- Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells.- Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy.- Evaluation of microRNA Delivery in vivo.- Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer.- Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data.- Construction of Multi-potent microRNA Sponge and its Functional Evaluation.- microRNA Sequencing Data Analysis Toolkits.

Textul de pe ultima copertă

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“The recent discovery of microRNAs … has represented a fundamental turning point in our understanding of gene expression. … In this book … the focus has been placed on this particular topic with a particularly strong emphasis towards ‘wet lab’ and computational approaches. … each chapter is nicely presented with detailed stepwise protocols that can be easily followed without any previous experience with microRNA research … . In summary, this is a book that I would recommend for any researcher to have on the shelf.” (Emanuele Buratti, Molecular Biotechnology, February, 2010)
“This well-written, well-illustrated, and concise book provides cutting-edge information on microRNA and its applications to cancer research from scientists around the world. … It is written for graduate students, postdoctoral fellows, and scientists in cancer research, and it is well suited for clinical oncologists and others in the field. … This book will be of immense help for … researchers in the field of cancer research.” (Omer Iqbal, Doody’s Review Service, March, 2011)
“Offers a compilation of reviews in the emerging field of microRNAs in cancer … . these reviews are concise and informative and provide a starting point to key aspects of this rapidly evolving field. … Each chapter ends with a helpful notes section and a comprehensive list of references for the applications discussed. … this book would be a valuable resource, both for those researchers considering the venture of entering this field, as well as for researchers already working in the field.” (Daniela Sanchez Basseres, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)