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Molecular Profiling: Methods and Protocols de Virginia Espina

Molecular Profiling: Methods and Protocols: Methods in Molecular Biology, cartea 823

Editat de Virginia Espina, Lance A. Liotta
en Limba Engleză Hardback – 12 noi 2011
The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling : Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling.

 Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.
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Specificații

ISBN-13: 9781603272155
ISBN-10: 1603272151
Pagini: 447
Ilustrații: XIII, 447 p.
Dimensiuni: 178 x 254 x 33 mm
Greutate: 0.98 kg
Ediția:2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

Tumor Staging and Grading: A Primer.-Clinical Trial Design in the Age of Molecular Profiling.-Personalized Medicine: Ethics for Clinical Trials.-Reduction of Preanalytical Variability in Specimen Procurement for Molecular Profiling.-The Human Side of Cancer Biobanking.-Introduction to Genomics.-Genomic Profiling: cDNA arrays and oligoarrays.-Genome wide methylation profiling in archival formalin fixed paraffin embedded (FFPE) tissue samples.-An Overview of microRNA Methods: Expression Profiling and Target Identification.-Antibody Validation by Western Blotting.-Laser Capture Microdissection: ArcturusXT infra-red capture and UV cutting methods.-Antibody microarrays: Analysis of Cystic Fibrosis.-Tissue Microarrays As A Tool In The Discovery & Validation Of Predictive Biomarkers.-Reverse phase protein microarrays.-Serum Low Molecular Weight Protein Fractionation for Biomarker Discovery.-Mass spectrometry based biomarker discovery.-Mitochondrial Proteome:  Toward The Detection and Profiling of Disease Associated Alterations.-Adult Neural Stem Cells: Isolation and Propagation.-Evanescent Wave Imaging: An Introduction to Total Internal Reflection Fluorescence Microscopy (TIRF).-Construction and Hyperspectral Imaging of Quantum Dot Lysate Arrays.-Microarray Data Analysis: Comparing Two Population Means.-Bioinformatics/Biostatistics: microarray analysis.-Structure-based functional design of drugs: from target to lead compound.-Personalized Medicine: Changing the Paradigm of Drug Development.-Grant Writing Tips for the First Time Applicant.-Inventions and Patents: A practical tutorial.-Regulatory Approval Pathways for Molecular Diagnostic Technology.-Small business development for molecular diagnostics.

Recenzii

From the reviews:
“This informative book combines genomics, proteomics, and bioinformatics with experimental protocols and detailed discussions regarding future challenges in commercialization and practical use in the field with an emphasis on tissue-based molecular profiling. … readers can use this as a practical guide at the laboratory bench as well as in the boardroom. The book is written for scientists, pathologists, oncologists, biotechnologists, residents, medical students, and nurses involved in clinical trial research. … This will be a valuable tool for those involved in clinical trial research.” (Omer Iqbal, Doody’s Book Reviews, April, 2012)

Textul de pe ultima copertă

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling: Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling.

 Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.

Caracteristici

Emphasis placed on tissue based molecular profiling not covered in pathology text books Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras