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NMR-Based Metabolomics: Methods and Protocols de G. A. Nagana Gowda

NMR-Based Metabolomics: Methods and Protocols: Methods in Molecular Biology, cartea 2037

Editat de G. A. Nagana Gowda, Daniel Raftery
en Limba Engleză Paperback – 29 aug 2020
This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advancesin the field of metabolomics.
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Specificații

ISBN-13: 9781493996926
ISBN-10: 1493996924
Pagini: 489
Ilustrații: XIV, 489 p. 123 illus., 96 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.86 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges.- Analysis of Plasma, Serum, and Whole Blood Metabolites Using 1H NMR Spectroscopy.- Lipid Profiling Using 1H-NMR Spectroscopy.- High Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics.- Practical Guidelines for 13C-Based NMR Metabolomics.- Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D 1H NMR Spectroscopy.- UHPLC-QTOF-MS/MS-SPE-NMR: A Solution to the Metabolomics Grand Challenge of Higher-Throughput, Confident Metabolite Identifications.- Fast NMR Methods for Identification of Resonances and Metabolic Pathways.- Stable Isotope-Resolved Metabolomics by NMR.- Tracking Metabolic Rewiring of Cancer Metabolism in Humans Using Isotope-Resolved NMR.- NMR-Based Metabolomics in Cardiac Research.- Breast Cancer Metabolomics Using NMR.- NMR-Based Urinary Metabolomics Applications.- NMR-Based Metabolomics in Gallbladder Cancer Research.- Biomarker Discovery Using NMR-Based Metabolomics of Tissue.- NMR Metabolomics Protocols for Drug Discovery.- Practical Aspects of the Analysis of Low- and High-Field NMR Data from Environmental Samples.- NMR-Based Metabolomics of Food.- Plant Metabolomics Using NMR Spectroscopy.- Fast Quantitative 2D NMR for Untargeted and Targeted Metabolomics.- Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization.- Metabolic Profiling Using In Vivo High Field Flow NMR.- Tools for Enhanced NMR-Based Metabolomics Analysis.- Automated Tools for the Analysis of 1D and 2D NMR Spectra.- Processing and Analysis of Untargeted Multi-Cohort NMR Data.- Advanced Statistical Methods for NMR-Based Metabolomics.

Textul de pe ultima copertă

This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advancesin the field of metabolomics.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts