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PCR Primer Design de Chhandak Basu

PCR Primer Design: Methods in Molecular Biology, cartea 1275

Editat de Chhandak Basu
en Limba Engleză Paperback – 8 oct 2016
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
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Specificații

ISBN-13: 9781493947553
ISBN-10: 1493947559
Pagini: 216
Ilustrații: XII, 216 p. 73 illus., 45 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.41 kg
Ediția:Softcover reprint of the original 2nd ed. 2015
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes.- Primer Design for PCR Reactions in Forensic Biology.- Design of Primers and Probes For Quantitative Real-Time PCR Methods.- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus.- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability.- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens.- Degenerate Primer Design for Highly Variable Genomes.- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection.- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers.- In silico PCR Primer Designing and Validation.- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR.- Designing Primers for SNaPshot Technique.- Rapid and Simple Method of qPCR Primer Design.- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design.- Selecting Specific PCR Primers with MFEprimer.

Textul de pe ultima copertă

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts