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Peptide Conjugation: Methods and Protocols: Methods in Molecular Biology, cartea 2355

Editat de Waleed M. Hussein, Rachel J. Stephenson, Istvan Toth
en Limba Engleză Paperback – 15 aug 2022
This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Peptide Conjugation: Methodsand Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. 

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Specificații

ISBN-13: 9781071616192
ISBN-10: 1071616196
Ilustrații: XII, 324 p. 79 illus., 59 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.59 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Peptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Conjugation of Peptides to Gold Nanoparticles.- Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus.- Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides.- Modification of Nanoparticles with Transferrin for Targeting Brain Tissues.- Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Vitamin B12 - Peptide Nucleic Acid Conjugates.- Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides.- Solid-State, Thermal Synthesis of Peptide/Protein–Boron Cluster Conjugates.- Bioconjugation of Peptides to Hybrid Gold Nanoparticles.- Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2.- Electrochemically-Enabled C-Terminal Peptide Modifications.- Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates.- Chemical Protein Synthesis by Chemoselective α-Ketoacid–Hydroxylamine (KAHA) Ligations with 5-Oxaproline.- Site-Specific Modification of Single-Chain Affinity Ligands for Fluorescence Labeling, Radiolabeling, and Bioconjugation.- Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags.- The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library.- The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation.- Synthesis of Lipopeptides by CLipPA Chemistry.- Constraining TAT Peptide by γPNA Hairpin for Enhanced Cellular Delivery of Biomolecules.- Preparation of mRNA Polyplexes with Post-Conjugated Endosome-Disruptive Peptides.- Targeted Subcellular Protein Delivery Using Cleavable CyclicCell-Penetrating Peptide-Conjugates.- Facile Chemoselective Modification of Thio-Ethers Generates Chiral Center-Induced Helical Peptides.

Textul de pe ultima copertă

This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Peptide Conjugation: Methods and Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. 


Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts