Peptide Research Protocols: Endothelin: Methods in Molecular Biology, cartea 206

Endothelin Peptide Protocols.- Immunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting Enzymes.- Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay.- Separation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by Radioimmunoassay.- Endothelin Receptor Protocols.- Radioligand binding assays and quantitative autoradiography of endothelin receptors.- Using Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin Receptor.- Quantification of Endothelin Receptor mRNA by Competitive RT-PCR.- Endothelin-Converting Enzyme Protocols.- Detection and Assessment of Endothelin-Converting Enzyme Activity.- Quantitative Measurement of mRNA Levels by RT-PCR.- Evaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells.- Endothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In Vitro.- In Vitro Functional Assays.- Characterization of a New Endothelin Receptor Ligand by In Vitro Assays.- Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation.- In Vivo Functional Assays.- Investigation of the Endothelin System in Experimental Heart Failure.- Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo.

"This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..." -Acta Cardiologica

Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.

Includes supplementary material: sn.pub/extras