Plant Cell and Tissue Culture: Methods in Molecular Biology, cartea 6

Selection of Media for Tissue and Cell Culture.- Growth Determination and Medium Analysis.- Measurements of Viability Suitable for Plant Tissue Cultures.- Cryopreservation of Plant Cells.- Hormonal Control of Growth and Development.- The Initiation and Maintenance of Callus Cultures.- Organogenesis in Callus Culture.- Manipulation of Shoot Formation in Cultured Explants.- Meristem-Tip Culture.- Micropropagation.- Grafting In Vitro.- Flower Organ Culture.- Fruit Organ Cultures.- Culture of Zygotic Embryos of Higher Plants.- Induction of Embryogenesis in Callus Culture.- Induction of Embryogenesis in Suspension Culture.- Microspore Culture in Brassica.- Growth of Ferns from Spores in Axenic Culture.- Clonal Propagation of Orchids.- Tissue Culture and Top-Fruit Tree Species.- Fertilization In Vitro and Culture of Fertilized Ovules of Higher Plants.- Vegetative Propagation of Cacti and Other Succulents In Vitro.- The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization.- Protoplasts of Higher and Lower Plants.- Plant Protoplast Enucleation by Density Gradient Centrifugation.- Isolation and Transplantation of Nuclei into Plant Protoplasts.- Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro.- Agrobacterium Rhizogenes-Mediated Gene Transfer Using PRI 1855 and a Binary Vector.- Transformation of Rape with Agrobacterium tumeficiens-Based Vectors.- Transient and Stable Expression of Foreign DNA Introduced into Plant Protoplasts by Electroporation.- Protoplast Microinjection Using Agarose Microdrops.- Transformation of Plants Via the Shoot Apex.- Methods of Gene Transfer and Analysis in Higher Plants.- Electrofusion of Plant Cells.- Production of Cybrids in Rapeseed (Brassica napus).- Selection of Somatic Hybrids by Resistance Complementation.- Dual Fungal and Plant Cell Culture.- Mutagenesis Techniques in Plant Tissue Cultures.- Mutagenesis.- Techniques for Selecting Mutants from Plant Tissue Cultures.- Selection of Antimetabolite Resistant Mutants.- Selection of Chloroplast Mutants.- Large-Scale Culture of Plant Cells.- Batch Suspension Culture.- Production Systems.- Immobilization of Cells by Spontaneous Adhesio.- Plant Cell Immobilization in Alginate and Polyurethane Foam.- Alkaloid Secondary Products from Catharanthus roseus Cell Suspension.- Betanin Production and Release In Vitro from Suspension Cultures of Beta vulgaris.- Enzyme Extraction and Assessment of Enzyme Production.- Micromachines to Automate Plant Tissue Culture.- Automation of the Surface Sterilization System Procedure.

Valuable for...its excellent step-by-step recipe approach and...as a comprehensive source book of practical guidance.-Journal of Plant Physiology