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Plant Cytogenetics: Methods and Protocols: Methods in Molecular Biology, cartea 1429

Editat de Shahryar F. Kianian, Penny M. Avoles Kianian
en Limba Engleză Hardback – 10 aug 2016
This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics. 
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Specificații

ISBN-13: 9781493936205
ISBN-10: 1493936204
Pagini: 212
Ilustrații: XII, 198 p. 40 illus., 32 illus. in color.
Dimensiuni: 178 x 254 x 13 mm
Greutate: 0.59 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-ColorFISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers forHigh-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: FluorescenceIn Situ Hybridization for IdentifyingHomologous Chromosomes.- Localizationof Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabelingand In Situ Labelling of IsolatedPlant Interphase Nuclei.- Manipulationof Homologous Chromosome Recombination in Wheat.- DissectingPlant Chromosomes by the Use of Ionizing Radiation.- OpticalNano-Mapping and Analysis of Plant Genomes.- FlowSorting Plant Chromosomes.- Constructionof BAC Libraries from Flow-Sorted Chromosomes.- TheChromosome Microdissection and Microcloning Technique.- Immunolocalizationon Whole Anther Chromosome Spreads for Male Meiosis.- MappingRecombination Initiation Sites Using Chromatin Immunoprecipitation.- ChromatinImmunoprecipitation to Study Plant Epigenome.

Notă biografică

Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108 
Email: Shahryar.Kianian@ars.usda.gov

Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108  
Email:P.Kianian@gmail.com 

Textul de pe ultima copertă

This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics.
 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras