Plant-Pathogen Interactions: Methods in Molecular Biology, cartea 354
Editat de Pamela C. Ronalden Limba Engleză Hardback – 22 noi 2006
Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.
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Paperback (1) | 638.11 lei 43-57 zile | |
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Humana Press Inc. – 22 noi 2006 | 645.47 lei 43-57 zile |
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Specificații
ISBN-13: 9781588294487
ISBN-10: 158829448X
Pagini: 248
Ilustrații: XII, 248 p. 24 illus.
Dimensiuni: 155 x 235 x 22 mm
Greutate: 0.56 kg
Ediția:2007
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 158829448X
Pagini: 248
Ilustrații: XII, 248 p. 24 illus.
Dimensiuni: 155 x 235 x 22 mm
Greutate: 0.56 kg
Ediția:2007
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
ResearchCuprins
The Use of Protoplasts to Study Innate Immune Responses.- Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae.- Whole-Genome Analysis to Identify Type III-Secreted Effectors.- In planta Expression of Oomycete and Fungal Genes.- Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice.- Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea.- Identification of Components in Disease-Resistance Signaling in Arabidopsis by Map-Based Cloning.- Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response.- Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses.- Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies.- Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response.- Use of Microarray Analysis to Dissect the Plant Defense Response.- Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions.- Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing.- Use of RNA Interference to Dissect Defense-Signaling Pathways in Rice.- Virus-Induced Gene Silencing in Plant Roots.- Methods for Engineering Resistance to Plant Viruses.- Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner.
Recenzii
From the reviews:
"In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. … The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. … this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers." (Dr Luis Mur, Molecular Biotechnology, 2007)
"The book is divided into 18 chapters … . this book is an excellent reference for people interested in validating or improving existing lab protocols. … serve as a basic reference work for those entering into a new research area. … Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money." (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008)
"In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. … With contributions from over 40 experts in their fields, this stepwise set of protocols … will certainly be of interest to molecular plant-pathogen researchers." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)
"In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. … The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. … this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers." (Dr Luis Mur, Molecular Biotechnology, 2007)
"The book is divided into 18 chapters … . this book is an excellent reference for people interested in validating or improving existing lab protocols. … serve as a basic reference work for those entering into a new research area. … Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money." (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008)
"In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. … With contributions from over 40 experts in their fields, this stepwise set of protocols … will certainly be of interest to molecular plant-pathogen researchers." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)
Caracteristici
Key methods, approaches, and strategies to dissect plant-pathogen interactions Discussion of the utility of viral induced gene silencing and RNAi silencing Readily reproducible protocols for the analysis of nucleic acids by nonradioactive methods Step-by-step instructions that ensure successful results Includes supplementary material: sn.pub/extras