Plant Vacuolar Trafficking: Methods and Protocols: Methods in Molecular Biology, cartea 1789
Editat de Cláudia Pereiraen Limba Engleză Hardback – 19 iun 2018
Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field
Toate formatele și edițiile | Preț | Express |
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Paperback (1) | 773.88 lei 38-45 zile | |
Springer – 10 dec 2019 | 773.88 lei 38-45 zile | |
Hardback (1) | 637.00 lei 6-8 săpt. | |
Springer – 19 iun 2018 | 637.00 lei 6-8 săpt. |
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Specificații
ISBN-13: 9781493978557
ISBN-10: 1493978551
Pagini: 205
Ilustrații: XII, 222 p. 51 illus., 41 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.63 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 1493978551
Pagini: 205
Ilustrații: XII, 222 p. 51 illus., 41 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.63 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Cuprins
Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds.- Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture.- Vacuolar Sorting Determinants – Isolation and Study.- Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies.- Plant Cell Vacuoles: Staining and Fluorescent Probes.- Vacuolar Targeting and Characterization of Recombinant Antibodies.- Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus.- Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells.- Live cell Imaging and Confocal Microscopy.- Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM).- The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana.- Use of Brefeldin A and Wortmannin to Dissect Post-Golgi OrganellesRelated to Vacuolar Transport in Arabidopsis thaliana.- Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking.- Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay.- Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae.- Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment.
Textul de pe ultima copertă
This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential from reproducible results Contains key notes and implementation advice from the experts