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Population Epigenetics: Methods and Protocols de Paul Haggarty

Population Epigenetics: Methods and Protocols: Methods in Molecular Biology, cartea 1589

Editat de Paul Haggarty, Kristina Harrison
en Limba Engleză Paperback – 12 iul 2018
This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.
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Specificații

ISBN-13: 9781493983339
ISBN-10: 1493983334
Pagini: 206
Ilustrații: XII, 206 p.
Dimensiuni: 178 x 254 mm
Greutate: 0.39 kg
Ediția:Softcover reprint of the original 1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Library Construction for High-Throughput Mobile Element Identification and Genotyping.- The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements.- Determining Epigenetic Targets: A Beginner’s Guide to Identifying Genome Functionality Through Database Analysis.- Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C.- Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method.- 5-Hydroxymethylcytosine Profiling in Human DNA.- Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach.- Correcting for Sample Heterogeneity in Methylome-Wide Association Studies.- Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations.- Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine.- Analysis of Imprinted Gene Regulation.- Statistical Methods for Methylation Data.

Textul de pe ultima copertă

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.

Caracteristici

Includes cutting-edge methods and protocols for the study of population epigenetics Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts