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Reporter Gene Imaging: Methods and Protocols: Methods in Molecular Biology, cartea 1790

Editat de Purnima Dubey
en Limba Engleză Hardback – 2 iun 2018
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and  bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații

ISBN-13: 9781493978588
ISBN-10: 1493978586
Pagini: 215
Ilustrații: XI, 221 p. 53 illus., 42 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.63 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function.- Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging.- Optical Bioluminescence Protocol for Imaging Mice.- Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase.- Reporter Based BRET Sensors for Measuring Biological Functions In Vivo.- Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains.- In vivo Bacterial Imaging using Bioluminescence.- Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging.- Hypoxia-induced Reporter Genes with Different Half-lives.- Visualization of Immune System Reconstitution by Bioluminescent Imaging.- Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo.- Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes.- Imaging T Cell Dynamics and Function byPET using Human Nuclear Reporter Genes.- Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT.- Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes.- Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models.

Textul de pe ultima copertă

This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and  bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts