Cantitate/Preț
Produs

Retinal Degeneration: Methods and Protocols: Methods in Molecular Biology, cartea 935

Editat de Bernhard H.F. Weber, Thomas Langmann
en Limba Engleză Paperback – 23 aug 2016
Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the  cutting-edge techniques of retinal cell biology in health and disease.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 101840 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 101840 lei  6-8 săpt.
Hardback (1) 72221 lei  6-8 săpt.
  Humana Press Inc. – 13 noi 2012 72221 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 101840 lei

Preț vechi: 107200 lei
-5% Nou

Puncte Express: 1528

Preț estimativ în valută:
19499 20304$ 16178£

Carte tipărită la comandă

Livrare economică 14-28 februarie

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493959693
ISBN-10: 1493959697
Pagini: 384
Ilustrații: XIV, 384 p. 98 illus., 48 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.69 kg
Ediția:Softcover reprint of the original 1st ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Identification and Analysis of Inherited Retinal Disease Genes.-Mouse Models for Studies of Retinal Degeneration and Diseases.-Retinal Fundus Imaging in Mouse Models of Retinal Diseases.-Functional Phenotyping of Mouse Models with ERG.-Phenotyping of Mouse Models with OCT.-Light Damage as a Model of Retinal Degeneration.-N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage – A Mouse Model of Acute Retinal Ganglion Cell Damage.-Generation of Transgenic X. laevis Models of Retinal Degeneration.-Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio.-Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking.-Analysis of the Drosophila Compound Eye with Light and Electron Microscopy.-Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy.-A Method of Horizontally-Sliced Preparation of the Retina.-Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL.-High-throughput RNA in situ Hybridization in Mouse Retina.- Assessment of Mitochondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity.-Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ.-Ca2+ Microfluorimetry in Retinal Müller Glial Cells.-Functional Analysis of Retinal Microglia and their Effects on Progenitors.-Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.-Ca2+ Imaging Techniques to Analyze Ca2+ Signaling in Cells and to Monitor Neuronal Activity in the Retina.-Double Chromatin Immunoprecipitation:Analysis of Target Co-Occupancy of Retinal Transcription Factors.-Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation.-Optimized Technique for Subretinal Injections in Mice.-Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations.-Barrier Modulation in Drug Delivery to the Retina.

Recenzii

From the book reviews:
“This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. … Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. … This book is great in simplifying new cutting-edge techniques in studying retinal degeneration.” (Ayham Skaf, Doody’s Book Reviews, May, 2013)

Textul de pe ultima copertă

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the  cutting-edge techniques of retinal cell biology in health and disease.

Caracteristici

Features a comprehensive overview and detailed lab protocols relevant to retinal homeostasis and disease Provides step-by-sep detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras