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Sepsis: Methods and Protocols de Wendy E. Walker

Sepsis: Methods and Protocols: Methods in Molecular Biology, cartea 2321

Editat de Wendy E. Walker
en Limba Engleză Paperback – 30 mai 2022
This detailed volume presents a variety of animal models that are commonly used to study sepsis and some key procedures to measure specific disease outcomes. The chapters describe well-established surgical and nonsurgical rodent models of sepsis, presented by experts in the field. In addition, the book includes protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which may be useful tools to increase the translational potential of rodent sepsis research. There is a chapter discussing the use of biomarkers for sepsis diagnosis and prognosis in humans and mice, as well as chapters describing specific methods for biomarker measurement, and the use of analgesics and humane endpoints in rodent sepsis research. Finally, agent-based computational modeling is presented as a valuable complementary approach to study sepsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Sepsis: Methods and Protocols serves as an ideal guide for researchers working toward improving the identification and treatment of septic patients.
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Specificații

ISBN-13: 9781071614907
ISBN-10: 1071614908
Pagini: 262
Ilustrații: XIII, 262 p. 53 illus., 50 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.49 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Cecal Ligation and Puncture.- Colon Ascendens Stent Peritonitis (CASP).- Induction of Sepsis via Fibrin Clot Implantation.- Cecal Slurry Injection in Neonatal and Adult Mice.- Injection of Escherichia coli to Induce Sepsis.- A Mouse Model of Pseudomonas aeruginosa Pneumonia.- A Mouse Model of Candidiasis.- Francisella tularensis Infection of Mice as a Model of Sepsis.- A Mouse Model of Necrotizing Enterocolitis.- A Murine Model of Full-Thickness Scald Burn Injury with Subsequent Wound and Systemic Bacterial Infection.- Mouse Intensive Care Unit (MICU).- Creation of BLT Humanized Mice for Sepsis Studies.- Scoring Sepsis Severity in Mice,0 Identification of ILC2 in the Lung Using Flow Cytometry.- Measurement of Intestinal Permeability During Sepsis.- Sepsis Biomarkers.- Detection of Blood Cell Surface Biomarkers in Septic Mice.- Microfluidic Chips for Sepsis Diagnosis.- Analgesia and Humane Endpoints for Rodents in Sepsis Research.- Agent-Based Modeling of Systemic Inflammation: A Pathway towards Controlling Sepsis.

Textul de pe ultima copertă

This detailed volume presents a variety of animal models that are commonly used to study sepsis and some key procedures to measure specific disease outcomes. The chapters describe well-established surgical and nonsurgical rodent models of sepsis, presented by experts in the field. In addition, the book includes protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which may be useful tools to increase the translational potential of rodent sepsis research. There is a chapter discussing the use of biomarkers for sepsis diagnosis and prognosis in humans and mice, as well as chapters describing specific methods for biomarker measurement, and the use of analgesics and humane endpoints in rodent sepsis research. Finally, agent-based computational modeling is presented as a valuable complementary approach to study sepsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Sepsis: Methods and Protocols serves as an ideal guide for researchers working toward improving the identification and treatment of septic patients.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts