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Spectral and Imaging Cytometry: Methods and Protocols de Natasha S. Barteneva

Spectral and Imaging Cytometry: Methods and Protocols: Methods in Molecular Biology, cartea 2635

Editat de Natasha S. Barteneva, Ivan A. Vorobjev
en Limba Engleză Paperback – 21 apr 2024
This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. 

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

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Specificații

ISBN-13: 9781071630228
ISBN-10: 1071630229
Pagini: 260
Ilustrații: XIV, 260 p. 1 illus.
Dimensiuni: 178 x 254 mm
Ediția:2nd ed. 2023
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Development of Spectral Imaging Cytometry.- Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer.- Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic Islands.- Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium.- Imaging Flow Cytometry of Multi-Nuclearity.- The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay.- High-Throughput γ-H2AX Assay Using Imaging Flow Cytometry.- Label Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry.- “Immuno-FlowFISH": Applications for Chronic Lymphocytic Leukaemia.- Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.- Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity.- Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF).- FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry.- Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospecies.

Textul de pe ultima copertă

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Cutting-edge and thorough, Spectral and Imaging Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. 

Chapters 1,2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts