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Staphylococcus Epidermidis: Methods and Protocols: Methods in Molecular Biology, cartea 1106

Editat de Paul D. Fey
en Limba Engleză Hardback – 13 noi 2013
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
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Specificații

ISBN-13: 9781627037358
ISBN-10: 1627037357
Pagini: 205
Ilustrații: X, 208 p. 30 illus., 18 illus. in color.
Dimensiuni: 178 x 254 x 20 mm
Greutate: 0.6 kg
Ediția:2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

S. epidermidis biology   1.  S. epidermidis disease (Mark Rupp) 2.  S. epidermidis pathogenesis/virulence factors (Michael Otto) 3.  Isolation of the organism in the clinical microbiology laboratory (?)   Molecular typing/population biology   4.  Genome of S. epidermidis (Steve Gill) 5.  Molecular epidemiology methodologies-PFGE, etc (not MLST) (Rich Goering) 6.  Population biology methodologies-MLST (Ashley Robinson)   Biofilm of S. epidermidis   7.  Methods to visualize and analyze biofilm formation in Staphylococcus epidermidis.  (Fey) 8.  Polysaccharide Intercellular Adhesin and biofilm formation in Staphylococcus epidermidis. 9.  Aap-mediated biofilm formation in S. epidermidis 10.  Biofilm formation mediated by Embp in S. epidermidis   Molecular Genetics and Tools   11.  Transposons and IS elements of S. epidermidis 12.  Allelic replacement methodologies in S. epidermidis. 13.  Transposon mutagenesis protocols and tools 14.  Transduction protocols in S. epidermidis   Animal Models of Infection   15.  Rat Catheter model (UNMC investigator) 16.  Mouse flank model (Tammy Kielian) 17.  Mouse orthopedic model (Carolyn, Mark, Kevin) 18.  Central Nervous System S. epidermidis shut model of infection (Snowden)​

Recenzii

From the reviews:
This 19-chapter book is entirely focused on everything you might ever want to know about Staphylococcus epidermidis. … This book would be of great interest to anyone working with Staphylococcus epidermidis, particularly those in a research setting. … many chapters come fully equipped with detailed experimental protocols ready for you to try in your laboratory. If you have any interest in any aspect of Staphylococcus epidermidis, you will want to read and likely own this book.” (Valerie L. Ng, Doody’s Book Reviews, February, 2014)

Textul de pe ultima copertă

Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades.  The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts