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Synthetic Biology de Karen M. Polizzi

Synthetic Biology: Methods in Molecular Biology, cartea 1073

Editat de Karen M. Polizzi, Cleo Kontoravdi
en Limba Engleză Hardback – sep 2013
 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.
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Specificații

ISBN-13: 9781627036245
ISBN-10: 1627036245
Pagini: 236
Ilustrații: X, 226 p. 80 illus., 48 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.59 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

What is Synthetic Biology?.- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program.- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit.- Overlap Extension PCR Cloning.- One-Step Isothermal Assembly of DNA Fragments.- Creation and Characterization of Component Libraries for Synthetic Biology.- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements.- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli.- Construction and Engineering of  Large Biochemical Pathways via DNA Assembler.- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors.- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research.- Combinatorial DNA Assembly using Golden Gate Cloning.- Construction of Synthetic Gene Circuits in the Escherichia coli Genome.- Shuffling of DNA Cassettes in a Synthetic Integrin.- Systematic Methodology for the Development of Mathematical Models for Biological Processes.-SOBOLHDMR: A General Purpose Modelling Software.

Textul de pe ultima copertă

 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Caracteristici

Reviews the latests developments in molecular biology techniques Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts