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Telomeres and Telomerase: Methods and Protocols: Methods in Molecular Biology, cartea 1587

Editat de Zhou Songyang
en Limba Engleză Hardback – 21 mar 2017
This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.
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Specificații

ISBN-13: 9781493968916
ISBN-10: 1493968912
Pagini: 348
Ilustrații: XI, 218 p. 37 illus., 18 illus. in color.
Dimensiuni: 178 x 254 x 14 mm
Greutate: 0.62 kg
Ediția:3rd ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Introduction to Telomeres and Telomerase.- Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9.- Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH).- Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH).- Telomere G-Rich Overhang Length Measurement: DSN Method.- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA.- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In.- Analysis of Yeast Telomerase by Primer Extension Assays.- Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP).- Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™).- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques.- Cytogenetic Analysis of Telomere Dysfunction.- Probing the Telomere Damage Response.- Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins.- Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein–Protein Interactions.- In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits.- Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.- Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization.- Analysis of Telomere Proteins by ChromatinImmunoprecipitation (ChIP). 
 

Textul de pe ultima copertă

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Recenzii

From the reviews of the second edition:
“The book provides different assays to study the function of telomere proteins and the responses of the telomere DNA. It also provides protocols for biochemical, molecular, and proteomic approaches in the understanding of telomere dynamics. It appears to be intended for investigators interested in the field of telomere and telomerase, but it also will be of immense help to other clinicians interested in cell growth, proliferation, aging, and cancer.” (Omer Iqbal, Doody’s Book Reviews, February, 2012)