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The Blood-Brain Barrier: Methods and Protocols de Nicole Stone

The Blood-Brain Barrier: Methods and Protocols: Methods in Molecular Biology, cartea 2492

Editat de Nicole Stone
en Limba Engleză Paperback – 25 iun 2023
This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
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Specificații

ISBN-13: 9781071622919
ISBN-10: 1071622919
Pagini: 357
Ilustrații: XI, 357 p. 83 illus., 73 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.64 kg
Ediția:1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease.- In Vitro Models of the Blood-Brain Barrier.- In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges.- Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial–Brain Endothelial Cell Interactions.- An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.- A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.- An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies.- Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury.- A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier.- A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress.- Novel, Emerging Chip Models of the BBB and Future Directions.- Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier.- Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels.- BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip.- Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability.- An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis.- An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke.- Immunohistochemical Analysis of Tight Junction Proteins.- An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells.- An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of β-Amyloid and Its Influence on PBMC Infiltration.

Textul de pe ultima copertă

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts