The Integrin Interactome: Methods and Protocols: Methods in Molecular Biology, cartea 2217
Editat de Miguel Vicente-Manzanaresen Limba Engleză Paperback – 20 noi 2021
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
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Paperback (1) | 691.04 lei 6-8 săpt. | |
Springer Us – 20 noi 2021 | 691.04 lei 6-8 săpt. | |
Hardback (1) | 940.68 lei 6-8 săpt. | |
Springer Us – 20 noi 2020 | 940.68 lei 6-8 săpt. |
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Specificații
ISBN-13: 9781071609644
ISBN-10: 1071609645
Pagini: 315
Ilustrații: XIX, 315 p. 54 illus., 43 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.59 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 1071609645
Pagini: 315
Ilustrații: XIX, 315 p. 54 illus., 43 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.59 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Cuprins
Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays.- Quantification of Integrin Activation and Ligation in Adherent Cells.- Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates.- Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads.- Functional Integrin Regulation through Interactions with Tetraspanin CD9.- Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners.- Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay.- Single-Protein Tracking to Study Protein Interactions during integrin-based Migration.- Biochemical Characterization of the Integrin Interactome.- Network Analysis of Integrin Adhesion Complexes.- Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions.- Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands andElectromagnetic Fields.- Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation.- Quantitative Analysis of Integrin Trafficking.- Methods to Study Integrin Functions on Exosomes.- Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome.- Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells.
Textul de pe ultima copertă
This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts