Cantitate/Preț
Produs
Total produse
Vezi coșul
The Mitotic Exit Network: Methods and Protocols de Fernando Monje-Casas

The Mitotic Exit Network: Methods and Protocols: Methods in Molecular Biology, cartea 1505

Editat de Fernando Monje-Casas, Ethel Queralt
en Limba Engleză Hardback – 9 noi 2016
This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods inMolecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 75658 lei  6-8 săpt.
  Springer – 27 iun 2018 75658 lei  6-8 săpt.
Hardback (1) 63269 lei  6-8 săpt.
  Springer – 9 noi 2016 63269 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 70% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 26096 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59855 lei
  • 19% Plant-Pathogen Interactions: Methods and Protocols
    Preț: 57771 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49678 lei
  • 20% Clinical Bioinformatics
    Preț: 88293 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 70873 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71049 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72009 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 72575 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 64446 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 99590 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 71354 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 88795 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 64558 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 63539 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 137406 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 72134 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 94440 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64158 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 71727 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 95666 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 71229 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39257 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 72490 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 93503 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86019 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 63395 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 102521 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79215 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42361 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42590 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59219 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 5% Synthetic Metabolic Pathways: Methods and Protocols
    Preț: 37270 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103882 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 206129 lei
  • 5% Epitope Mapping Protocols
    Preț: 126192 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78992 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133907 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 135031 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75265 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37488 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 135552 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 109722 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 136778 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 109261 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 93855 lei

Preț: 63269 lei

Preț vechi: 74433 lei
-15% Nou

Puncte Express: 949
Preț estimativ în valută:
12110€ • 12621$ • 10081£

Carte tipărită la comandă

Livrare economică 07-21 ianuarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493965007
ISBN-10: 149396500X
Pagini: 281
Ilustrații: X, 281 p. 35 illus., 24 illus. in color.
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.72 kg
Ediția:1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Regulation of Mitotic Exit in Saccharomyces cerevisiae.- Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression.- Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining.- Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators.- Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release.- In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP.- Analysis of Protein-Protein Interactions Between MEN Components.- A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity.- Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain.- A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets.- Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast.- Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.- Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP).- Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies.- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays.- Analysis of the Functionality of the Mitotic Checkpoints.- Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions.- Studying the Role of the Mitotic Exit Network in Cytokinesis.- Hippo Signaling in Mitosis: An Updated View in Light of the MEN Pathway.

Textul de pe ultima copertă

This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.

Caracteristici

Includes cutting-edge methods and protocols for studying the Mitotic Exit Network Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts