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The Nuclear Receptor Superfamily: Methods and Protocols: Methods in Molecular Biology, cartea 1443

Editat de Iain J. McEwan, PhD
en Limba Engleză Paperback – 26 mai 2018
Thisvolume aims to describe acomplementary range of molecular, cell biological, and in vivo protocols used to investigatethe structure–function of nuclear receptors, together with experimentalapproaches that may lead to new drugs to selectively target nuclearreceptor-associated diseases. The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritativeand cutting-edge, The Nuclear ReceptorSuperfamily, Second Edition aims to ensure successful results in thefurther study of this vital field.
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Specificații

ISBN-13: 9781493981205
ISBN-10: 149398120X
Ilustrații: X, 250 p. 44 illus., 32 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.46 kg
Ediția:Softcover reprint of the original 2nd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

The Nuclear Receptor Superfamily- AtThirty.- Lipid Homeostasis and Ligands forLiver X Receptors: Identification and Characterization.- Useof Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for NovelInhibitors of the Androgen Receptor.- Useof BRET to Study Protein-Protein Interactions in vitro and in vivo.- StudyingSteroid Receptor Complexes in the Cellular Environment.- Post-translationalModifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to IdentifyChromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated byAndrogens.- Methods for Identifying andQuantifying Mrna Expression of Androgen Receptor Splicing Variants in ProstateCancer.- HarvestingHuman Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- Invivo Imaging of Nuclear Receptor Transcriptional Activity.- Developmentand Characterisation of Cell-Specific Androgen Receptor Knockout Mice.




Textul de pe ultima copertă

This volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure–function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts