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Therapeutic Peptides: Methods and Protocols: Methods in Molecular Biology, cartea 1088

Editat de Andrew E. Nixon
en Limba Engleză Hardback – 22 oct 2013
Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage.  With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Practical and dependable,  Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.
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Specificații

ISBN-13: 9781627036726
ISBN-10: 1627036725
Pagini: 265
Ilustrații: XI, 279 p. 53 illus., 8 illus. in color.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.68 kg
Ediția:2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Design of Phage-Displayed Cystine-Stabilized Mini-Protein Libraries for Proteinaceous Binder Engineering.- Construction of a Filamentous Phage Display Peptide Library.- Engineering Bio-Active Peptide-Based Therapeutic Molecules.- T7 Lytic Phage-Displayed Peptide Libraries: Construction and Diversity Characterization.- Affinity Selection Using Filamentous Phage Display.- Bioprospecting Open Reading Frames for Peptide Effectors.- Identification of Ideal Peptides for Heterovalent Ligands.- Substrate Phage Display for Protease Substrate Sequence Characterization: Bovine Factor Xa as a Model System.- Engineering Peptide Therapeutics Using MIMETIBODY™ Technology.- Evaluation of Peptides as Protease Inhibitors and Stimulators.- Assessment of Antimicrobial (Host Defense) Peptides as Anti-Cancer Agents.- Peptide Labelling Strategies for Imaging Agents.- Peptide Optimization and Conjugation Strategies in the Development of Molecularly Targeted Magnetic Resonance Imaging Contrast Agents.- Evaluation of Prenylated Peptides for Use in Cellular Imaging and Biochemical Analysis.- Ultraviolet Absorption Spectroscopy of Peptides.- Fluorescence Spectroscopy of Peptides.- Circular Dichroism of Peptides.- Fourier Transform Infrared Spectroscopy of Peptides.

Textul de pe ultima copertă

Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage.  With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Practical and dependable,  Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.

Caracteristici

Features methods for the identification of peptide therapeutic candidates through a wide variety of techniques Includes step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts