Thyroid Hormone Receptors: Methods and Protocols: Methods in Molecular Biology, cartea 202
Editat de Aria Baniahmaden Limba Engleză Hardback – 4 iun 2002
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Specificații
ISBN-13: 9780896039957
ISBN-10: 0896039951
Pagini: 223
Ilustrații: X, 223 p.
Dimensiuni: 155 x 235 x 21 mm
Greutate: 0.51 kg
Ediția:2002
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 0896039951
Pagini: 223
Ilustrații: X, 223 p.
Dimensiuni: 155 x 235 x 21 mm
Greutate: 0.51 kg
Ediția:2002
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
Professional/practitionerCuprins
to Thyroid Hormone Receptors.- Null Mutant Mice for Thyroid Hormone Receptors.- Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray.- The Role of Thyroid Hormone Receptors in the Heart.- Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization.- The v-erbA Oncogene.- Gene Regulation by Thyroid Hormone Receptors.- Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome.- Function of Thyroid Hormone Receptors During Amphibian Development.- Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin.- Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex.
Textul de pe ultima copertă
Because thyroid hormone receptors (TRs) are important regulators of a large number of biological processes-ranging from brain development to control of vision in vertebrates-abnormal functioning of these receptors can have serious medical consequences. In Thyroid Hormone Receptors: Methods and Protocols, a panel of outstanding investigators surveys and explains the major cutting-edge methods used in TR research and their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TRs in development and generation of knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for the study of blood cell differentiation through the oncogene v-erbA, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver. Each protocol has been developed by a hands-on expert and contains full explanatory notes, troubleshooting tips, and helpful comments on avoiding pitfalls.
Broad ranging and highly practical, Thyroid Hormone Receptors: Methods and Protocols offers endocrinologists, geneticists, biochemists, and research physicians a comprehensive collection of powerful methods useful not only for all major areas of TR biology and analysis, but also for nuclear hormone receptors in general.
Broad ranging and highly practical, Thyroid Hormone Receptors: Methods and Protocols offers endocrinologists, geneticists, biochemists, and research physicians a comprehensive collection of powerful methods useful not only for all major areas of TR biology and analysis, but also for nuclear hormone receptors in general.
Caracteristici
Includes supplementary material: sn.pub/extras