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Transgenic Maize: Methods and Protocols: Methods in Molecular Biology, cartea 526

Editat de M. Paul Scott
en Limba Engleză Paperback – 19 noi 2010
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
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Specificații

ISBN-13: 9781617379352
ISBN-10: 1617379352
Pagini: 200
Ilustrații: IX, 188 p.
Dimensiuni: 210 x 280 x 11 mm
Greutate: 0.45 kg
Ediția:2009
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Design of Gene Constructs for Transgenic Maize.- Transformation Methods.- Transient Expression of GFP in Immature Seed Tissues.- Biolistic Gun-Mediated Maize Genetic Transformation.- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus.- Whiskers-Mediated Maize Transformation.- Transgenic Maize in Research.- Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines.- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression.- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites.- Analysis of Transgenic Plants.- Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize.- Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds.- Determination of Transgene Copy Number by Real-Time Quantitative PCR.- Breeding with Transgenes.- Herbicide Resistance Screening Assay.- Characterizing Transgene Inheritance.- Backcross Breeding.

Recenzii

From the reviews:
“This book is number 526 in the popular ‘Methods in Molecular Biology’ series, which is renowned for its targeted protocol chapters, each with photographs, recipes and a set of notes for additional practical guidance and to avoid common pitfalls. … There is a lot to like about this collection of practical chapters, which represent an excellent investment for the applied maize researcher, who could be a student doing an undergraduate project through to a professional scientist in a dedicated transformation laboratory.” (Huw D. Jones, Annals of Botany, Vol. 109 (7), June, 2012)

Textul de pe ultima copertă

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Caracteristici

Contains a comprehensive set of methods for developing transgenic maize varieties, including construct design, transformation, analysis, and breeding Includes methods in tissue culture, molecular biology, biochemistry, genetics, and agronomy Presents data from case studies of research with transgenic maize Appendices provide examples of regulatory paperwork required by the US government for experimental production of transgenic maize Displays full-color plates to illustrate the utility of fluorescent marker proteins Allows readers to compare transformation methods used by public and private sector laboratories