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Tumor Angiogenesis Assays: Methods and Protocols de Domenico Ribatti

Tumor Angiogenesis Assays: Methods and Protocols: Methods in Molecular Biology, cartea 2572

Editat de Domenico Ribatti
en Limba Engleză Paperback – 28 sep 2023
This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
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Paperback (2) 75496 lei  6-8 săpt.
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Specificații

ISBN-13: 9781071627051
ISBN-10: 1071627058
Ilustrații: XI, 212 p. 55 illus., 49 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.4 kg
Ediția:2nd ed. 2023
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Discovery and development of tumor angiogenesis assays.- The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino.- Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry.- Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels.- Visualizing the interactions that haping the imaging of microenvironment in cancer diseases.- Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach.- Monitoring angiotropic extravascular migratory metastasis in vitro.- Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option.- Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment.- IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM).- Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model.- The chick CAM as an in vivo system to study stem cell activity.- Detection of possible symmetries in vascular networks by computer assisted image analysis.- Alternative in vivo models to study teratoma.- How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation.- 3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models.

Textul de pe ultima copertă

This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts