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Type-1 Diabetes: Methods and Protocols de Kathleen M. Gillespie

Type-1 Diabetes: Methods and Protocols: Methods in Molecular Biology, cartea 1433

Editat de Kathleen M. Gillespie
en Limba Engleză Hardback – 3 iun 2016
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.


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Specificații

ISBN-13: 9781493936410
ISBN-10: 1493936417
Pagini: 276
Ilustrații: X, 213 p. 44 illus., 33 illus. in color.
Dimensiuni: 178 x 254 x 14 mm
Greutate: 0.61 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Type 1 Diabetes: Prediction And Prevention: Current Perspectives.- The HLA.- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.- Islet Autoantibody Analysis: Radioimmunoassays.- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.- Histology of the Type 1 Diabetes Pancreas.- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.- FISH with Concomitant Immunofluorescence.- Laser Capture Microdissection:  Single Pancreatic Cell DNA Profiling.- The Gut Microbiome in the NOD Mouse.- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes. 


 

Textul de pe ultima copertă

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras