Cantitate/Preț
Produs
Total produse
Vezi coșul
Wnt Signaling: Methods and Protocols de Quinn Barrett

Wnt Signaling: Methods and Protocols: Methods in Molecular Biology, cartea 1481

Editat de Quinn Barrett, Lawrence Lum
en Limba Engleză Hardback – 3 sep 2016
Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 68993 lei  6-8 săpt.
  Springer – 9 iun 2018 68993 lei  6-8 săpt.
Hardback (1) 64662 lei  6-8 săpt.
  Springer – 3 sep 2016 64662 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 64662 lei

Preț vechi: 76073 lei
-15% Nou

Puncte Express: 970
Preț estimativ în valută:
12374€ • 12784$ • 10293£

Carte tipărită la comandă

Livrare economică 20 martie-03 aprilie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493963911
ISBN-10: 1493963910
Pagini: 163
Ilustrații: XI, 198 p. 45 illus., 32 illus. in color.
Dimensiuni: 178 x 254 x 13 mm
Greutate: 0.59 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Visualizing Wnt Palmiteoylation in Single Cells.- Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction.- Biochemical Methods to Analyze Wnt Protein Secretion.- Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum.- Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway.- A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells.- Wnt-Dependent Control of Cell Polarity in Cultured Cells.- The Use of Chick Embryos to Study Wnt Signaling/Gradients.- Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.- Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo.- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts.- Delivery of the Porcupine Inhibitor WNT974 in Mice.- Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis.- Monitoring Wnt/b-Catenin Signaling in Skin.- The Generation of Organoids for Studying Wnt Signaling.- Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells.- Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.

Textul de pe ultima copertă

Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.

Caracteristici

Includes cutting-edge methods and protocols for the study of Wnt signaling Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts