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Yeast Protocols: Methods in Molecular Biology, cartea 2196

Editat de Wei Xiao
en Limba Engleză Paperback – 6 sep 2021
This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
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Specificații

ISBN-13: 9781071608708
ISBN-10: 1071608703
Pagini: 260
Ilustrații: XI, 260 p. 55 illus., 44 illus. in color.
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.48 kg
Ediția:4th ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

 Use of Yeast Plasmids - Transformation and Inheritance Assays.- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae.- CRISPR Nickase-Mediated Base Editing in Yeast.- Genomic Promoter Shuffling by Using Recyclable Cassettes.- Study Essential Gene Functions by Plasmid Shuffling.- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae.- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe.- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map.- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE.- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering.- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9.- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA.- Assays for Autophagy III: Observing Dynamic Protein Trafficking.- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification.- High-Throughput Rapid Yeast Chronological Lifespan Assay.- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast.- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis. 

Textul de pe ultima copertă

This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community. 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From Reviews of the First Edition...

"...will benefit the apprentices...and the experienced scientist venturing into a new technique or changing yeast species...should join the other 'cookery' books on the laboratory shelf." -Journal of Emergency Medicine

"...a useful addition to life sciences libraries and to laboratories embarking on studies with yeast"
-Quarterly Review of Biology