Biomembrane Protocols: II. Architecture and Function: Methods in Molecular Biology, cartea 27
Autor John M. Graham, Joan A. Higginsen Limba Engleză Paperback – 11 sep 2013
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Specificații
ISBN-13: 9781489939098
ISBN-10: 1489939091
Pagini: 380
Ilustrații: XIII, 362 p. 7 illus.
Dimensiuni: 152 x 229 x 20 mm
Greutate: 0.51 kg
Ediția:1994
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 1489939091
Pagini: 380
Ilustrații: XIII, 362 p. 7 illus.
Dimensiuni: 152 x 229 x 20 mm
Greutate: 0.51 kg
Ediția:1994
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
ResearchCuprins
Crystallization of Membrane Proteins for X-Ray Analysis.- Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination.- Biochemical Methods to Determine Cell-Surface Topography.- Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins.- The Production of Monoclonal Antibodies to Membrane Proteins.- Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles.- Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy.- Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy.- Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes.- Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools.- Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry.- Fluorescent Glycerolipid Probes.- Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids.- Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes.- Extraction and Assay of Cyclic Nucleotides.- Analysis of G-Proteins Regulating Signal Transduction Pathways.- Assay of Protein Kinases and Protein Phosphorylation.- Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures.- Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography.- Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes.- Membrane Permeabilization with Bacterial Toxins.- Measurement of Ion Fluxes and pH Gradients Across Cell Membranes.- Ligand Binding andProcessing.- The Binding of Protein-Ligands to Cell-Surface Receptors.
Recenzii
...the editors have assembled an authoritative set of reviews, which give generally clear practical guidance and useful methodological tips. -SGM Quarterly